PANA RealTyper™ (PNA probe-based fluorescence melting curve analysis) is based on the change in fluorescent signal as a sample thermally denatured (melting temperature shift). PANA RealTyper™ is a powerful tool for mutation detection (screening and genotyping) by a single probe. PANA RealTyper™ can distinguish multiple SNPs adjacent to each other on a short target region. This method offers easy and simple data analysis without using analyzing software because of its clarity of melting peak. PANA RealTyper™ can be applied in the field of personalized medicine, origin discrimination and detection of multidrug-resistant microorganisms.